Coding

Part:BBa_K1189014:Design

Designed by: Somshukla Chaudhuri   Group: iGEM13_Calgary   (2013-09-17)

GFP fused to an E coil under a lacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

This part has been designed in the RFC 25 Freiburg fusion backbone and can be used to make fusion proteins. The ATG codon is incorporated in the backbone before the NgoMIV restriction cut site.

References

  • Litowski, J. R., & Hodges, R. S. (2002). Designing heterodimeric two-stranded alpha-helical coiled-coils. Effects of hydrophobicity and alpha-helical propensity on protein folding, stability, and specificity. The Journal of Biological Chemistry, 277(40), 37272–9. doi:10.1074/jbc.M204257200